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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
17/10/2018 |
Actualizado : |
11/03/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ZARZA, R.; REBUFFO, M.; LA MANNA, A.; BALZARINI, M. |
Afiliación : |
RODRIGO TABARE ZARZA FUENTES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO LA MANNA ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MÓNICA BALZARINI, Universidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias, Córdoba, Argentina. |
Título : |
Plant density in red clover (Trifolium pratense L.) pastures as an early predictor of forage production. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
European Journal of Agronomy, November 2018, v. 101, p. 193-199. Doi: https://doi.org/10.1016/j.eja.2018.10.004 |
DOI : |
10.1016/j.eja.2018.10.004 |
Idioma : |
Inglés |
Notas : |
Article history: Received 6 March 2018// Received in revised form 3 October 2018// Accepted 8 October 2018. |
Contenido : |
ABSTRACT.-
Red clover (Trifolium pratense L.) is an alternative of great potential productivity for dairy systems, particularly in heavily compacted soils. Plant density (PD) during pasture establishment can be related to forage production and used as an early indicator of pasture quality. However, biomass predictive models for red clover are not readily available. To predict red clover biomass a few weeks after sowing would help farmers to adopt suitable management practices. The aim of this paper has therefore been to model the relationship between red clover biomass and PD at different time points during pasture establishment to identify the best monitoring moment for
estimating future herbage productivity. A multi-environment trial, including several seeding rates simulating different levels of establishment within each of the nine environments, was conducted in Uruguay. Seedlings were counted 3, 7 and 12 weeks after sowing (WS). Biomass of first-cut (C1) harvest was linearly related to PD at
7 WS, whereas a second-order polynomial on PD at 7 WS was a significant predictor of accumulated biomass one (Y1) and two (Y2) years after sowing. PD at 3 WS was a strong predictor of biomass only in high-yielding environments. In such environments, more than 64 plants m?2 at 3 WS suggest a high probability of achieving
annual yields above 10,000 kg DM ha-1. Therefore, early PD determination (3 or 7 WS) is a good indicator of annual productivity in pure red clover pastures. |
Palabras claves : |
DENSIDAD DE PLANTAS; FORAGE BIOMASS; GRASSLAND; PASTURE ESTABLISHMENT; PASTURES; PLANT DENSITY; PREDICTIVE MODEL; PRODUCCIÓN FORRAJERA. |
Thesagro : |
FORRAJES; LEGUMINOSAS; PASTURAS; TREBOL ROJO; TRIFOLIUM PRATENSE. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02604naa a2200337 a 4500 001 1059208 005 2021-03-11 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1016/j.eja.2018.10.004$2DOI 100 1 $aZARZA, R. 245 $aPlant density in red clover (Trifolium pratense L.) pastures as an early predictor of forage production.$h[electronic resource] 260 $c2018 500 $aArticle history: Received 6 March 2018// Received in revised form 3 October 2018// Accepted 8 October 2018. 520 $aABSTRACT.- Red clover (Trifolium pratense L.) is an alternative of great potential productivity for dairy systems, particularly in heavily compacted soils. Plant density (PD) during pasture establishment can be related to forage production and used as an early indicator of pasture quality. However, biomass predictive models for red clover are not readily available. To predict red clover biomass a few weeks after sowing would help farmers to adopt suitable management practices. The aim of this paper has therefore been to model the relationship between red clover biomass and PD at different time points during pasture establishment to identify the best monitoring moment for estimating future herbage productivity. A multi-environment trial, including several seeding rates simulating different levels of establishment within each of the nine environments, was conducted in Uruguay. Seedlings were counted 3, 7 and 12 weeks after sowing (WS). Biomass of first-cut (C1) harvest was linearly related to PD at 7 WS, whereas a second-order polynomial on PD at 7 WS was a significant predictor of accumulated biomass one (Y1) and two (Y2) years after sowing. PD at 3 WS was a strong predictor of biomass only in high-yielding environments. In such environments, more than 64 plants m?2 at 3 WS suggest a high probability of achieving annual yields above 10,000 kg DM ha-1. Therefore, early PD determination (3 or 7 WS) is a good indicator of annual productivity in pure red clover pastures. 650 $aFORRAJES 650 $aLEGUMINOSAS 650 $aPASTURAS 650 $aTREBOL ROJO 650 $aTRIFOLIUM PRATENSE 653 $aDENSIDAD DE PLANTAS 653 $aFORAGE BIOMASS 653 $aGRASSLAND 653 $aPASTURE ESTABLISHMENT 653 $aPASTURES 653 $aPLANT DENSITY 653 $aPREDICTIVE MODEL 653 $aPRODUCCIÓN FORRAJERA 700 1 $aREBUFFO, M. 700 1 $aLA MANNA, A. 700 1 $aBALZARINI, M. 773 $tEuropean Journal of Agronomy, November 2018$gv. 101, p. 193-199. Doi: https://doi.org/10.1016/j.eja.2018.10.004
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
17/04/2024 |
Actualizado : |
17/04/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. |
Afiliación : |
ANA PAULA AREVALO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; SERGIO ANCHETA, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; KAREN PERELMUTER, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; ALEJANDRO RICCIARDI; MARIELA BOLLATI-FOGOLÍN, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; MARTINA CRISPO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay. |
Título : |
3R's applied to in vivo biological activity of recombinant human erythropoietin assay. |
Complemento del título : |
Technical Article. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. |
ISSN : |
2675-9225 |
DOI : |
10.4322/2675-9225.00012023 |
Idioma : |
Inglés |
Notas : |
Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. --
Publisher: Brazilian Society for Laboratory Animal Science. |
Contenido : |
ABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. |
Palabras claves : |
3R’s; Biological activity; Erythropoietin; European Pharmacopeia; Mice; UNIDAD MIXTA PASTEUR + INIA. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
https://bmrtsbcaljournal.com/article/10.4322/2675-9225.00012023/pdf/bmrt-3-1-e00012023.pdf
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Marc : |
LEADER 02721naa a2200301 a 4500 001 1064596 005 2024-04-17 008 2023 bl uuuu u00u1 u #d 022 $a2675-9225 024 7 $a10.4322/2675-9225.00012023$2DOI 100 1 $aAREVALO, A.P. 245 $a3R's applied to in vivo biological activity of recombinant human erythropoietin assay.$h[electronic resource] 260 $c2023 500 $aArticle history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. -- Publisher: Brazilian Society for Laboratory Animal Science. 520 $aABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. 653 $a3R’s 653 $aBiological activity 653 $aErythropoietin 653 $aEuropean Pharmacopeia 653 $aMice 653 $aUNIDAD MIXTA PASTEUR + INIA 700 1 $aBASIKA, T. 700 1 $aANCHETA, S. 700 1 $aPERELMUTER, K. 700 1 $aRICCIARDI, A. 700 1 $aBOLLATI-FOGOLÍN, M. 700 1 $aCRISPO, M. 773 $tBiological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS.
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